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Literature summary extracted from
- Comprehensive characterization of secreted aspartic proteases encoded by a virulence gene family in Candida albicans (2011), J. Biochem., 150, 431-438.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.23.24 | expressed in Pichia pastoris strain GS115 | Candida albicans |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.23.24 | additional information | isozyme Sap7 is not inhibited by pepstatin A, leupeptin, phenylmethylsulfonyl fluoride and EDTA | Candida albicans | |
| 3.4.23.24 | pepstatin A | isozymes 1-10 except 7 | Candida albicans |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.24 | 11000 | - |
1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE | Candida albicans |
| 3.4.23.24 | 15000 | - |
1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE | Candida albicans |
| 3.4.23.24 | 35000 | - |
isozyme Sap8, calculated from amino acid sequence | Candida albicans |
| 3.4.23.24 | 36000 | - |
isozyme Sap1, calculated from amino acid sequence | Candida albicans |
| 3.4.23.24 | 36000 | - |
isozyme Sap2, calculated from amino acid sequence | Candida albicans |
| 3.4.23.24 | 36000 | - |
isozyme Sap5, estimated from SDS-PAGE | Candida albicans |
| 3.4.23.24 | 36000 | - |
isozyme Sap8, estimated from SDS-PAGE | Candida albicans |
| 3.4.23.24 | 37000 | - |
isozyme Sap3, SDS-PAGE and calculated from amino acid sequence | Candida albicans |
| 3.4.23.24 | 37000 | - |
isozyme Sap4, calculated from amino acid sequence | Candida albicans |
| 3.4.23.24 | 37000 | - |
isozyme Sap5, calculated from amino acid sequence | Candida albicans |
| 3.4.23.24 | 37000 | - |
isozyme Sap6, calculated from amino acid sequence | Candida albicans |
| 3.4.23.24 | 38000 | - |
isozyme Sap2, estimated from SDS-PAGE | Candida albicans |
| 3.4.23.24 | 38000 | - |
isozyme Sap6, estimated from SDS-PAGE | Candida albicans |
| 3.4.23.24 | 39000 | - |
isozyme Sap1, estimated from SDS-PAGE | Candida albicans |
| 3.4.23.24 | 39000 | - |
isozyme Sap4, estimated from SDS-PAGE | Candida albicans |
| 3.4.23.24 | 42000 | - |
isozyme Sap10, estimated from SDS-PAGE | Candida albicans |
| 3.4.23.24 | 44000 | - |
isozyme Sap9, estimated from SDS-PAGE | Candida albicans |
| 3.4.23.24 | 44000 | - |
1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE | Candida albicans |
| 3.4.23.24 | 45000 | - |
isozyme Sap10, calculated from amino acid sequence | Candida albicans |
| 3.4.23.24 | 47000 | - |
isozyme Sap7, calculated from amino acid sequence | Candida albicans |
| 3.4.23.24 | 52000 | - |
isozyme Sap7, estimated from SDS-PAGE | Candida albicans |
| 3.4.23.24 | 52000 | - |
1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE | Candida albicans |
| 3.4.23.24 | 53000 | - |
isozyme Sap9, calculated from amino acid sequence | Candida albicans |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.23.24 | Candida albicans | - |
- |
- |
| 3.4.23.24 | Candida albicans | P0CY27 | - |
- |
| 3.4.23.24 | Candida albicans | P0CY29 | - |
- |
| 3.4.23.24 | Candida albicans | P0DJ06 | - |
- |
| 3.4.23.24 | Candida albicans | P43094 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.23.24 | glycoprotein | isozymes Sap4, 6, 8, 9 and 10 are N-glycosylated. Sap7 occurs in glycosylated forms other than N-glycosylation | Candida albicans |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.23.24 | anti-FLAG M2 affinity gel filtration | Candida albicans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.23.24 | casein + H2O | isozyme Sap6 shows highest activity towards casein | Candida albicans | ? | - |
? | |
| 3.4.23.24 | FRETS-25Ala + H2O | isozyme Sap1 shows highest activity towards FRETS-25Ala | Candida albicans | ? | - |
? | |
| 3.4.23.24 | FRETS-25Xaa + H2O | all Sap isozymes prefer Arg and Lys at the Xaa position | Candida albicans | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.23.24 | heterodimer | 1 * 44000 + 1 * 11000, isozyme Sap9, SDS-PAGE | Candida albicans |
| 3.4.23.24 | heterodimer | 1 * 52000 + 1 * 15000, isozyme Sap7, SDS-PAGE | Candida albicans |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.23.24 | SAP | - |
Candida albicans |
| 3.4.23.24 | SAP1 | isozyme | Candida albicans |
| 3.4.23.24 | Sap10 | isozyme | Candida albicans |
| 3.4.23.24 | SAP2 | isozyme | Candida albicans |
| 3.4.23.24 | SAP3 | isozyme | Candida albicans |
| 3.4.23.24 | SAP4 | isozyme | Candida albicans |
| 3.4.23.24 | SAP5 | isozyme | Candida albicans |
| 3.4.23.24 | SAP6 | isozyme | Candida albicans |
| 3.4.23.24 | Sap7 | isozyme | Candida albicans |
| 3.4.23.24 | Sap8 | isozyme | Candida albicans |
| 3.4.23.24 | Sap9 | isozyme | Candida albicans |
| 3.4.23.24 | secreted aspartic protease | - |
Candida albicans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.23.24 | 2.5 | - |
isozyme Sap8 | Candida albicans |
| 3.4.23.24 | 3 | - |
isozyme Sap3 | Candida albicans |
| 3.4.23.24 | 4 | - |
isozyme Sap2 | Candida albicans |
| 3.4.23.24 | 5 | - |
isozyme Sap1 | Candida albicans |
| 3.4.23.24 | 5 | - |
isozyme Sap4 | Candida albicans |
| 3.4.23.24 | 5 | - |
isozyme Sap5 | Candida albicans |
| 3.4.23.24 | 5 | - |
isozyme Sap6 | Candida albicans |
| 3.4.23.24 | 5.5 | - |
isozyme Sap9 | Candida albicans |
| 3.4.23.24 | 6 | - |
isozyme Sap10 | Candida albicans |
| 3.4.23.24 | 6.5 | - |
isozyme Sap7 | Candida albicans |
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